GC-MS ANALYSIS OF CUTICULAR HYDROCARBON PROFILING ON ANOPHELES GAMBIAE MOSQUITOES EXPOSED TO MELON LEAF EXTRACT

Authors

  • Oluwadamilola Aina Confluence university of science and technology, osara, Kogi state
  • Upahi L Department of Biology, Applied Entomology and Parasit-ology Unit, Confluence University of Science and Technology Osara, Kogi – State , Nigeria Confluence University of Science and Technology Osara, Kogi State
  • Olayemi I. K Federal University of Technology, Minna, Niger State
  • Ukubuiwe A. C Federal University of Technology, Minna, Niger State
  • Abubakar N. E Federal University of Technology, Minna, Niger State
  • Abdullahi N. M Department of Biology, Applied Entomology and Parasit-ology Unit, Confluence University of Science and Technology Osara, Kogi – State , Nigeria Confluence University of Science and Technology Osara, Kogi State

DOI:

https://doi.org/10.67601/njbls.v3i1.20

Keywords:

Cuticular hydrocarbon, GC-MS, Anopheles Mosquitoes, Melon Leaf Extract, Sublethal Concentration

Abstract

Anopheles mosquito are vector of malaria, a deadly disease that affects millions of people worldwide. Developing effective and sustainable methods for mosquito control is crucial in malaria prevention efforts. This study investigates the potential of melon leaf extract as natural alternative for disrupting the cuticular hydrocarbon (CHCs) profile of mosquitoes, which may play a vital role in the behaviour of mosquitoes including desiccation resistance, communication, and species recognition. Altering these hydrocarbon can affect mosquito survivability and vector competence, making it a significant area of reseach in vector control. Melon leaf extract, known for its diverse bioactive compounds, was hypothesized to modify CHCs profile of anopheles mosquitoes. Adults mosquitoes were exposed to sub lethal dose under controlled conditions, and their cuticles were subsequently analysed using GC-MS. The results of these study demonstrated that exposure to melon leaf extract significantly altered the CHC profile of the mosquitoes. Notable changes were observed in the abundance and presence of specific hydrocarbon chains, including alkanes, alkenes and methyl-branched hydrocarbons. The control group of this study revealed a total number of 78 compounds in mosquitoes reared in 100 % distilled water while 97 compounds were encountered in mosquitoes reared 1 ml methanol solvent + 99 ml of distilled water respectively. The mosquitoes reared in sub lethal concentration of methanol melon leaf extract revealed 20 compounds. These alteration suggest potential in the mosquito physiological processess, which could influence their ability to thrive and transmit diseases.
KEYWORDS: cuticular hydrocarbon, GC-MS, Anopheles mosquitoes, melon leaf extract and sublethal concentration.

Downloads

Published

2026-06-15

Issue

Vol. 3 No. 1 (2026)

Section

Articles